Supercritical CO2 extraction of velutinol A from Mandevilla velutina (Apocynaceae) cultured cells and MALDI-TOF MS analysis

Maraschin M; Sugui JA; Wood KV; Bonham C; Lancas FM; Araujo PS; Yunes RA; Verpoorte R; Fontana JD

Biotechnology Letters, Vol.23, No.1, 77-82, 2001

DOI10.1023/A:1026776815646 Export Citation

Supercritical CO2 extraction of velutinol A from Mandevilla velutina (Apocynaceae) cultured cells and MALDI-TOF MS analysis

Maraschin M, Sugui JA, Wood KV, Bonham C, Lancas FM, Araujo PS, Yunes RA, Verpoorte R, Fontana JD

MALDI-TOF MS analysis of supercritical CO2 extracted samples obtained from Mandevilla velutina cell cultures allowed the detection of the anti-bradykinin pregnanic steroid, velutinol A, using low amount of sample (1 g lyophilized cells), with minimum analyte isolation.

Keywords:MALDI-TOF MS;Mandevilla velutina;secondary metabolites;supercritical fluid extraction;velutinol A