A 1 wt% lysozyme solution was used as a model fluid to test a previously formulated mathematical model for the initial chemical reaction fouling rate of a heat transfer surface. The experimental results showed that, at a given wall temperature, a maximum initial fouling rate existed over a range of fluid velocities. The maximum rate and the fluid velocity at which it occurred both increased as the wall temperature increased. These observations were consistent with the model. Quantitatively, the average absolute percent deviation between the experimental results and the optimum model predictions was 23.3%. The decrease in initial fouling tate with increasing velocity at high fluid velocities was even greater than predicted by the model.