Kinetics and Rate of Enzymatic Hydrolysis of Cellulose in Supercritical Carbon Dioxide

Chang Yeol Park; Yeon Woo Ryu; Chul Kim

Korean Journal of Chemical Engineering, Vol.18, No.4, 475-478, July, 2001

DOI10.1007/BF02698293 Export Citation

Kinetics and Rate of Enzymatic Hydrolysis of Cellulose in Supercritical Carbon Dioxide

Chang Yeol Park, Yeon Woo Ryu¹, and Chul Kim^{1, †}

Division of Chemical Engineering and Biotechnology, Ajou University, Suwon 442-749, Korea
¹Research Center for New Bio-Materials in Agriculture, Seoul National University, Suwon 441-744, Korea

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Experiments were carried out on the application of supercritical fluid to the hydrolysis of cellulose by the enzyme, cellulase. The stability of cellulase was sustained at the pressures of up to 160 atm for 90 min at 50 ℃ in supercritical carbon dioxide. In the hydrolysis of cellulose the glucose yield was 100% at supercritical condition. Kinetic constants of hydrolysis at supercritical condition were increased as compared to those at atmospheric condition. The hydrolysis reaction was found competitively inhibited by glucose at supercritical condition.

Keywords:Cellulase;Supercritical Carbon Dioxide;Kinetic Constant;Competitive Inhibition;Enzyme Stability

[References]

Blanch HW, Randolph TW, Chark DS, Prausnitz JM, Science, 239, 387 (1988)
Chi YM, Makamura K, Yano T, Agri. Biol. Chem., 52, 1541 (1988)
Dordick JS, Enzyme Microb. Technol., 11, 194 (1989)
Hammond DA, Karel M, Krukonis VJ, Appl. Biochem. Biotechnol., 11, 393 (1985)
Lee HK, Hong SI, HWAHAK KONGHAK, 25(2), 109 (1987)
Kim DW, Yang JH, Jung YG, HWAHAK KONGHAK, 24(5), 407 (1986)
Kamat SV, Beckman EJ, Russell AJ, Biotechnology, 15, 41 (1995)
Kasche V, Schlothauer R, Brunner G, Biotechnol. Lett., 10, 569 (1988)
Oh KD, Kim C, Korean J. Chem. Eng., 4(2), 105 (1987)
Lee HS, Lee WG, Park SW, Lee H, Chang HN, Biotechnol. Tech., 7, 267 (1993)
Lee HS, Ryu YW, Kim C, J. Microbiol. Biotechnol., 4, 230 (1994)
Marty A, Chulalaksanukul W, Wilemot RM, Condoret JS, Durand G, Biotechnol. Lett., 12, 11 (1990)
Nakamura K, Chi YM, Yano T, Chem. Eng. Commun., 45, 207 (1985)
Rafi ZK, Jonathan SD, Alexander MK, Biotechnol. Bioeng., 28, 417 (1986)
Randolph TW, Blanch HW, Prausnitz JM, Wilke CR, Biotechnol. Lett., 7, 325 (1985)
Taniguchi T, Kamihira M, Kobayashi T, Agric. Biol. Chem., 51, 593 (1987)
Zheng YZ, Lin HM, Wen JQ, Cao NJ, Yu XZ, Tsao GT, Biotechnol. Lett., 17(8), 845 (1995)
Zheng YZ, Tsao GT, Biotechnol. Lett., 18(4), 451 (1996)

[Referenced By]

[1] Blanch HW, Randolph TW, Chark DS, Prausnitz JM, Science, 239, 387 (1988)

[2] Chi YM, Makamura K, Yano T, Agri. Biol. Chem., 52, 1541 (1988)

[3] Dordick JS, Enzyme Microb. Technol., 11, 194 (1989)

[4] Hammond DA, Karel M, Krukonis VJ, Appl. Biochem. Biotechnol., 11, 393 (1985)

[5] Lee HK, Hong SI, HWAHAK KONGHAK, 25(2), 109 (1987)

[6] Kim DW, Yang JH, Jung YG, HWAHAK KONGHAK, 24(5), 407 (1986)

[7] Kamat SV, Beckman EJ, Russell AJ, Biotechnology, 15, 41 (1995)

[8] Kasche V, Schlothauer R, Brunner G, Biotechnol. Lett., 10, 569 (1988)

[9] Oh KD, Kim C, Korean J. Chem. Eng., 4(2), 105 (1987)

[10] Lee HS, Lee WG, Park SW, Lee H, Chang HN, Biotechnol. Tech., 7, 267 (1993)

[11] Lee HS, Ryu YW, Kim C, J. Microbiol. Biotechnol., 4, 230 (1994)

[12] Marty A, Chulalaksanukul W, Wilemot RM, Condoret JS, Durand G, Biotechnol. Lett., 12, 11 (1990)

[13] Nakamura K, Chi YM, Yano T, Chem. Eng. Commun., 45, 207 (1985)

[14] Rafi ZK, Jonathan SD, Alexander MK, Biotechnol. Bioeng., 28, 417 (1986)

[15] Randolph TW, Blanch HW, Prausnitz JM, Wilke CR, Biotechnol. Lett., 7, 325 (1985)

[16] Taniguchi T, Kamihira M, Kobayashi T, Agric. Biol. Chem., 51, 593 (1987)

[17] Zheng YZ, Lin HM, Wen JQ, Cao NJ, Yu XZ, Tsao GT, Biotechnol. Lett., 17(8), 845 (1995)

[18] Zheng YZ, Tsao GT, Biotechnol. Lett., 18(4), 451 (1996)