Biotechnology Letters, Vol.18, No.9, 1007-1012, 1996
Metabolism of Meta-Chlorinated or/and Para-Chlorinated Benzoic-Acids by the Crude Extract of Alcaligenes Sp Strain Cpe3 Grown on 3,4-Dichlorobenzoic Acid
A crude extract of Alcaligenes sp. CPE3 strain grown on 3,4-dichlorobenzoic acid metabolised 3- and 4-chlorobenzoic acid by reactions requiring O-2 and NADH, and 3,4-dichlorobenzoic acid by a reaction requiring O-2, NADH, FAD and FMN. The specific activity of the extract vs. 3-chlorobenzoic acid was described by the Michaelis-Menten kinetics, that vs. 4-chlorobenzoic acid was described by a substrate inhibitory kinetics and that vs. 3,4-dichlorobenzoic acid exhibited a two-peaked profile.
Keywords:PSEUDOMONAS-CEPACIA 2CBS;BACTERIAL CULTURE;PUTIDA P111;DEGRADATION;2;4-DICHLOROBENZOATE;2-CHLOROBENZOATE;CHLOROBENZOATES;1;2-DIOXYGENASE