HWAHAK KONGHAK, Vol.24, No.1, 53-62, February, 1986
셀룰로오스 및 Plant Biomass의 효소적 가수분해
Enzymatic Hydrolysis of Cellulose and Plant Biomass
초록
여러 종류의 셀룰로오스 및 plant biomass의 효소에 의한 가수분해에 대하여 논의하였다. Trichoderma viride, cellulase에 의한 이들 물질의 가수분해는 0.15 M, pH 5.0, 아세트산 완충용액에서 50 ℃로 이루어졌다. 또한 가수분해의 최적조건은, 100 mg 셀룰로오스에 대하여 25 mg의 셀룰라아제 및 반응시간에 있어서 72시간임을 발견하였다. 실험을 통하여 얻은 글루코오스의 최대수율은, cellulose native에 대하여 반응시간 72시간에서 % saccharification으로서 82.7 %였다.
This paper deals with the enzymatic hydrolysis of different kinds of celluloses and plant biomasses. The enzymatic hydrolysis of these materials by a complete cellulase from Trichoderma viride were carried out in the 0.15M acetate buffer solution at pH 5.0, 50 ℃.
The optimum hydrolysis conditions of cellulose were found 25 mg cellulase, from Trichoderma viride for 100 mg cellulose and 72 hours. The maximum yield of glucose for cellulose native from these experiments was 82.7 % in per cent saccharification for 72 hours.
The optimum hydrolysis conditions of cellulose were found 25 mg cellulase, from Trichoderma viride for 100 mg cellulose and 72 hours. The maximum yield of glucose for cellulose native from these experiments was 82.7 % in per cent saccharification for 72 hours.