The efficiency of the application of crude and purified MnP in processes such as degradation of hazardous compounds is greatly dependent of the Mn2+ and H2O2 concentrations. Mn2+ exerted a positive effect on the reaction rate whereas excessively high H2O2 concentrations caused a partial inactivation of MnP, and as a result additional increases of Mn2+ did not positively affect DMP oxidation rate. According to our results, concentrations around 5,000 mu M Mn2+ and 100 mu M H2O2 would maximize the catalytic MnP properties for the oxidation of 2,6-dimethoxyphenol. The presence of other cofactors in the crude enzyme such as organic acids stabilize formed Mn3+ and the oxidation rate was higher than the corresponding to the purified one.