Biotechnology Letters, Vol.19, No.7, 669-673, 1997
Apoptosis Extent Increases in Hybridoma Cultures with Temperature
Apoptosis plays an important role in determining efficacies of bioreactors employing hybridoma cells. Exposure to a 42 degrees C shock for 1 h increased the apoptosis extent (DNA fragmentation) by 32% in CCSC10 hybridoma. Further, glutamine at 2 mM decreased temperature-induced apoptosis by about 20%.