HWAHAK KONGHAK, Vol.24, No.4, 263-267, August, 1986
Cellulase에 의한 Plant Cellulose의 분해 및 알코올 발효
Degradation of Plant Cellulose by Cellulase and Alcohol Fermentation
초록
여러 종류의 plant cellulose의 효소에 의한 가수분해에 대하여 논의하였다. Trichoderma viride로부터 얻은 cellulase에 의한 이들 물질의 가수분해는, 0.15 M, pH 5.0, 아세트산 완충용액에서 50 ℃로 이루어졌으며, 가수분해의 조건은 100 mg plant cellulose에 대하여, 25 mg의 셀룰라아제 및 반응시간에 있어서 72시간이었다. 실험을 통하여 얻은 글루코오스의 최대 수율은, 반응시간 72시간에서, % saccharification으로써 옥수수대에 대하여 37.4 %였다. 그리고 효모를 사용하여, 이들 글루코오스를 알코올발효시켜 이론치의 95-98 %의 에틸알코올을 얻었다.
This paper deals with the enzymatic hydrolysis of different kinds of plant celluloses. The enzymatic hydrolysis of these materials by a complete cellulase from Trichoderma viride were carried out in the 0.15 M acetate buffer solution at pH 5.0, 50 ℃, and hydrolysis conditions were 25 mg cellulase, from Trichoderma viride for 100 mg plant cellulose and 72 hours.
The maximum yield of glucose from these experiments was 37.4 % in per cent saccharification for the indian corn stem without pretreatment for 72 hours. This enzymatic hydrolyzed solution of indian corn stem was concentrated by means of reversed osmosis until 100 mg/ml glucose concentrations. And this glucose solution was fermented into ethanol, using the strain Saccharomyces carlsbergensis w34, giving yields of 95-98 % of the theoretical value.
The maximum yield of glucose from these experiments was 37.4 % in per cent saccharification for the indian corn stem without pretreatment for 72 hours. This enzymatic hydrolyzed solution of indian corn stem was concentrated by means of reversed osmosis until 100 mg/ml glucose concentrations. And this glucose solution was fermented into ethanol, using the strain Saccharomyces carlsbergensis w34, giving yields of 95-98 % of the theoretical value.