Biotechnology Letters, Vol.19, No.12, 1175-1179, 1997
Construction of a green-fluorescent protein-based, insertion-inactivation shuttle vector for lactic acid bacteria and Escherichia coli
A shuttle vector, p5aGFP2201a, for lactic acid bacteria and E. coli was constructed by using the gene of a jellyfish green fluorescent protein (gfp) as a selection marker. The plasmid was shown to function as a shuttle vector by its ability to carry and express a staphylococcal chloramphenicol acetyltransferase (cat) gene into targeted hosts.