Biotechnology Letters, Vol.20, No.1, 73-75, 1998
Conditioned medium from heterogeneous plants (rose and grape) on cell growth and anthocyanin synthesis of Fragaria ananassa
Rose and grape cells were cultured under 800 lux for 1 week, and these broths were used as conditioned medium for stimulate anthocyanin synthesis of strawberry suspension cells. Anthocyanin synthesis in strawberry cells was enhanced to produce 1000 mu g/g cells after 2 days of culturing in the conditioned medium prepared from white grape callus which does not produce anthocyanin, and the enhancement was significantly (p < 0.05) greatest and it was 5 fold greater than that of the control medium. Stimulation by conditioned media also showed a concentration-dependent response for anthocyanin synthesis, although there was only a slight difference (4.0-4.8 g) in cell growth among the heterogeneous conditioned media after 2 days of culturing. However after 5 days, cell growth using 50% of conditioned media from red grape callus was 14.6 g cell weight and 15 g in the control.