Regioselective synthesis of e.g. 6-O-phenylbutyryl-1-n-butyl-beta-D-glucopyranose was achieved in 21 % yield using almond-beta-glucosidase and Candida antarctica lipase B. The beta-glucosidase reaction was performed in a biphasic (buffer/n-alcohol) system using free and Eupergit C(TM)-immobilized glucosidase. Immobilized enzyme allowed product formation even at a water content of 1 %.