Bipolar membrane electrodialysis is applied to the acidification of sodium 2-keto-L-gulonate and sodium L-ascorbate to replace the original ion exchange acidification for sodium gulonate, and sulfuric acidification for sodium L-ascorbate in the production of Vitamin C. Technical feasibility and current efficiency is studied. Ordinary electrodialysis is applied to the removal of sodium sulfate in the final Vitamin C solution. Sodium sulfate can be effectively removed with little Vitamin C loss.