Biotechnology Letters, Vol.22, No.1, 43-45, 2000
The use of the replicating pDblet plasmid as a cloning vector with enhanced stability in Kluyveromyces marxianus
The replicating plasmid, pDblet, transformed the budding yeast Kluyveromyces marxianus to an efficiency of 10(4) transformant mu g(-1) DNA. Transformed cells showed 1% of segregation rate without affecting their growth rate of 0.69 h(-1) and glucose consumption. These results were similar or better than the commonly used pE1 plasmid and suggests that pDblet can be used for cloning genes in K. marxianus.