Chlorination of gloves has gained popularity as a more permanent method of reducing the inherent tackiness of natural rubber latex compared to using powder as a dusting lubricant. Transmission of proteins in natural rubber latex into the air as a result of using powder on natural rubber latex gloves may cause serious complications to allergic individuals. A methodology for characterizing commercial chlorinated natural rubber latex gloves using a combination of attenuated total reflectance (ATR)Fourier transform infrared (FTIR) spectroscopy and ion chromatography (IC) is described. ATR-FTIR studies established that 930-915 and 670-650 cm(-1) are definitive wavenumber ranges for the identification of chlorine in commercial chlorinated latex gloves. Confirmation of the ATR-FTIR results and semiquantification of the chlorine content in the latex gloves was carried out by the IC technique. This methodology can be used by glove manufacturers to determine the amount of chlorine in batches of commercial gloves, and thereby prevent possible threats to public health arising from the deterioration of surgical and examination chlorinated latex gloves under severe storage conditions before the end of their expected shelf life.