Enzyme and Microbial Technology, Vol.28, No.9-10, 792-795, 2001
An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate
beta -amylases are used in production of maltose syrup. It is shown that sweet potato beta -amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean and barley enzymes indicate that this may be a general method for purification of beta -amylases.