Alignment and orientation effects in the energy pooling process, Sr(5s5p P-3(1)) + Sr(5s5p P-3(1)) --> Sr(5s5p P-1(1)) + Sr(5s(2) S-1(0)), are studied in a single atomic beam. The Sr atoms are prepared in an aligned initial state with a polarized laser, and alignment information is extracted by observing the fluorescence from the final Sr(5s5p P-1(1)) state as the atoms precess in an applied magnetic field. This allows the dependence of the total energy pooling cross section (integrated over the final state alignment) on the initial state alignment to be almost completely described. A prominent alignment effect is observed. If m(j) represents the component of total electronic angular momentum of a Sr(5s5p P-3(1)) atom along the relative velocity vector of the collision, then the total energy transfer cross section for a particular initial state alignment can be expressed as a sum of the so-called fundamental cross sections, sigma(m1m2) and sigma(m1m2;m'm2'), that describe collisions between the various m(j) states. Here sigma(m1m2) represents the cross section for energy transfer when an atom in state m(1) collides with an atom in state m(2). The cross section sigma(m1m2;m1'm2') represents the contribution to the total cross section from interference when the colliding system is in a superposition of the state m(1) colliding with m(2) and the state m(1)' colliding with m(2)'. It is found that the cross sections sigma(1-1), sigma(00), and sigma(10) as well as the interference terms Re(sigma(00;1-1)) and sigma(01;10) have relatively large values while sigma(11) and sigma(1-1;-11) are small. Coupled with future theoretical work, these results may provide new insights into the dynamics of the curve crossings that lead to energy transfer.