The relationship between membrane potential and flux of amino acid has been analyzed for the counter-transport system of amino acid coupled to the various concentration of added electrolytes through the sup-ported liquid membrane. The liquid membrane contained Aliquat 336 as liquid anion exchanger in various solvents with different dielectric constants. For the supported organic liquid membrane, solvents with lower dielectric constants was favored so that flux of amino acid was increased against it. The membrane potential decreased with increasing potentiometric selectivity se-quence of added electrolytes; however, the flux of amino acid increased. Above the detection limit the membrane potential increased with increasing concentration of added electrolytes; however, the flux of amino acid decreased.