폐수내 페놀의 생물학적 분해공정 Model에 관한 연구

이상영; 박부길; 이현용

HWAHAK KONGHAK, Vol.28, No.2, 172-176, April, 1990

Export Citation

폐수내 페놀의 생물학적 분해공정 Model에 관한 연구

Cometabolism Process for Biodegradation of Phenolic Substances

이상영, 박부길, 이현용

초록

폐수내 존재하는 적은 양의 유해물질이 미생물에 의해 분해시 cometabolism 과정에 의해 분해됨이 연속 및 회분배양으로 입증됐으며, phenol 분해시 유도된 bioenergetic 수율 model에 의한 0.1026(1/h)의 cometabolism coefficient와 0.587의 glucose최대 전환수율이 계산됐다. Pseudomonas sp. 의 생육을 저해하지 않은 phenol의 농도는 20㎍/㎖ 이하였으며, phenol유사체인 m-cresol로 미리 배양시킨 경우 phenol 분해능이 우수했으며 전체 균체량도 증가했다.

A bioenergetic model has been developed to estimate cometabolism coefficient along with mainte-nance coefficient for cometabolism process where small amounts of toxic materials exist with major carbon and energy source(eg. glucose) in the waste water. Cometabolism coefficient and maximum bioenergetic yield were estimated as 0.1026(1/h)and 0.585, respectively for the growth of Pseudomonas sp. under aerobic continuous cultivation. It also turned out that phenol was utilized as a cometabolic substrate where its concentration was below 20㎍/ml. Pre-cul-tivation with a phenol analog, m-cresol enhanced biodegradation rates of phenol and also increased total biomass concentration along with the extension of stationary phase.

[References]

Hansch LA, Elkins D, Chem. Rev., 71, 601 (1971)
Howard PH, Durkin P, "Sources of Contamination, Ambient Levels, and Fate of Benzene in the Environment," EPA report 560/5-75-005, Washington, DC (1975)
Pawlowski L, Klepacka B, Zalewski R, Water Res., 5, 1153 (1981)
Ghosal D, You I, Chatterjee DK, Chakrabarty AM, Science, 238, 135 (1985)
Tong RM, Beck M, Latten A, Automatica, 16, 659 (1980)
Stumm ZE, Appl. Microbiol., 14, 654 (1977)
Alexander M, Science, 211, 132 (1981)
Papanostsiu A, Maier W, Biotechnol. Bioeng., 24, 2001 (1982)
Hsieh JH, Wang SS, Enzyme Microb. Technol., 2, 299 (1982)
Dalton H, Stirling DI, Phil. Trans. R. Soc. London, 297, 481 (1982)
Hulbert MH, Krawiec S, J. Theor. Biol., 69, 287 (1977)
Grunt JK, Evans WC, Biochem. J., 79, 25 (1961)
Kilpi S, Backstron V, Korhola M, FEMS Microbiol. Lett., 18, 1 (1983)
Schmidt SK, Simkins SK, Alexander M, Appl. Environ. Microbiol., 50, 323 (1985)
Pawlowsky U, Howell JA, Biotechnol. Bioeng., 15, 889 (1973)
Edwards VH, Biotechnol. Bioeng., 12, 679 (1970)
Pirt SJ, Principles of Microbe and Cell Cultivation, Blackwell Sci. Pub., London (1975)
Erickson LE, Biotechnol. Bioeng., 21, 725 (1979)
Lee HY, Erickson LE, Yang SS, J. Ferment. Technol., 62, 341 (1984)
Shimp RJ, Pfaender FK, Appl. Environ. Microbiol., 49, 394 (1985)
Erickson LE, Biotechnol. Bioeng., 22, 1929 (1980)

[Referenced By]

[1] Hansch LA, Elkins D, Chem. Rev., 71, 601 (1971)

[2] Howard PH, Durkin P, "Sources of Contamination, Ambient Levels, and Fate of Benzene in the Environment," EPA report 560/5-75-005, Washington, DC (1975)

[3] Pawlowski L, Klepacka B, Zalewski R, Water Res., 5, 1153 (1981)

[4] Ghosal D, You I, Chatterjee DK, Chakrabarty AM, Science, 238, 135 (1985)

[5] Tong RM, Beck M, Latten A, Automatica, 16, 659 (1980)

[6] Stumm ZE, Appl. Microbiol., 14, 654 (1977)

[7] Alexander M, Science, 211, 132 (1981)

[8] Papanostsiu A, Maier W, Biotechnol. Bioeng., 24, 2001 (1982)

[9] Hsieh JH, Wang SS, Enzyme Microb. Technol., 2, 299 (1982)

[10] Dalton H, Stirling DI, Phil. Trans. R. Soc. London, 297, 481 (1982)

[11] Hulbert MH, Krawiec S, J. Theor. Biol., 69, 287 (1977)

[12] Grunt JK, Evans WC, Biochem. J., 79, 25 (1961)

[13] Kilpi S, Backstron V, Korhola M, FEMS Microbiol. Lett., 18, 1 (1983)

[14] Schmidt SK, Simkins SK, Alexander M, Appl. Environ. Microbiol., 50, 323 (1985)

[15] Pawlowsky U, Howell JA, Biotechnol. Bioeng., 15, 889 (1973)

[16] Edwards VH, Biotechnol. Bioeng., 12, 679 (1970)

[17] Pirt SJ, Principles of Microbe and Cell Cultivation, Blackwell Sci. Pub., London (1975)

[18] Erickson LE, Biotechnol. Bioeng., 21, 725 (1979)

[19] Lee HY, Erickson LE, Yang SS, J. Ferment. Technol., 62, 341 (1984)

[20] Shimp RJ, Pfaender FK, Appl. Environ. Microbiol., 49, 394 (1985)

[21] Erickson LE, Biotechnol. Bioeng., 22, 1929 (1980)