The novel cyclic voltammetric system was constructed using the Al and ITO electrode to analyze organic EL materials and the cyclic voltammetric control programs were also developed. The rubrene film was grafted on an ITO electrode and Al electrode by vacuum deposition. The three-electrode compartment electrochemical cell consisted of ITO and Al glass as the working electrode, a platinum wire as the counter electrode, and Ag/Ag+ (0.1M AgNO3) as the reference electrode. Comparison of the Ag/Ag+ reference electrode with the SCE reference electrode showed the potential of +0.36V at 5x10(-4)M ferrocene in 0.1M Bu4NClO4 with DME IP and EA values of rubrene were 5.48eV and 2.59eV, respectively, in 0.1M Bu4NClO4 with DMF.