We synthesized the new photo-alignment material, PM4Ch (poly (4-methacryloyloxy chalcone)). Good thermal stabilities of synthesized PM4Ch were obtained by TGA (Thermogravimetric Analysis) measurement. The electro-optical (EO) characteristics of the photo-aligned twisted nematic (TN)-LC display (LCD), with linearly polarized UV exposure of normal direction on the PM4Ch surface were investigated, Monodomain alignments of the NLC by polarized UV exposure of normal direction on the PM4Ch surface were observed. The excellent voltage- transmittance curves for the photo-aligned TN-LCD on the PM4Ch surfaces were successfully obtained. Also, a fast response time for the photo-aligned TN-LCD on the PM4Ch surface can be achieved. Reduction of the DC voltage for the photo-aligned TN-LCD decreases with increasing UV exposure time on the PM4Ch surfaces.