Two techniques, Matrix-Assisted Pulsed-Laser Evaporation (MAPLE) and MAPLE Direct Write (MDW) were developed to deposit biomaterial thin-films. MAPLE involves dissolving or suspending the biomaterial in a volatile solvent, freezing the mixture to create a solid target, and using a low fluence pulsed laser to evaporate the target for deposition of the solute inside a vacuum system. Using simple shadow masks, i.e. lines, dots and arrays, pattern features with length scales as small as 20 mum can be deposited using multiple materials on different types of substrates. MDW uses pulsed laser to directly transfer material from a ribbon to a substrate. Patterns with a spatial resolution of similar to 10 mum can be written directly. Biomaterials ranging from polyethylene glycol to eukaryotic cells, i.e. Chinese hamster ovaries, were deposited with no measurable damage to their structures or genotype. Deposits of immobilized horseradish peroxidase, an enzyme, in the form of a polymer composite with a protective coating, i.e. polyurethane, retained their enzymatic functions. A dopamine electrochemical sensor was fabricated by MDW using a natural tissues/graphite composite. These examples and the unique features of MAPLE and MDW for biosensor fabrication have been discussed.