화학공학소재연구정보센터
Biotechnology Letters, Vol.23, No.16, 1341-1343, 2001
Modification of the embryogenic response of Coffea arabica by the nitrogen source
Somatic embryogenesis was induced in coffee from in vitro cultured plants as starting material and the faster response obtained allowed lines from selected plants to be generated more quickly. In contrast to other systems, where embryos take 2 or 3 months to develop, globular embryos were obtained after 3 weeks. The optimum nitrogen concentrations for embryogenesis were between 3.75 and 15 mM nitrogen with a nitrate/ammonium molar ratio of 2:1 or 1:2.