This paper describes the results of a study on use of the adsorption-regeneration technique for recovery of dissolved allyl isothiocyanate (AITC) which is present in significant quantities in steam distillation condensate of mustard essential oil (MEO). Equilibrium and dynamic breakthrough studies were carried out. The adsorbents used were polymeric adsorbents: Amberlite XAD-2, Amberlite XAD-4, Amberlite XAD-7, NPA-1 and NPA-2. The results obtained indicate that Amberlite XAD-4 is the best amongst these various adsorbents. AITC recoveries in excess of 95% were obtained, indicating the feasibility of this approach. Further, since no chemical reactions are involved the recovered AITC retains its 'natural' origin.