A poly(glycidyl methacrylate-co-ethylene dimethacrylate) polymeric matrix was synthesized, amino-functionalized and packed to be tested for protein retention and separation. Functionalization was carried out by reacting the epoxy groups with 30% ammonia solution to provide amino groups for anion exchange with proteins. Physical characterization of the particulate material showed that the functionalized polymer is macroporous and exhibits a unimodal pore size distribution. The resin presents good thermal stability. Chromatographic characterization using bovine serum albumin (BSA) and alpha -lactalbumin as model proteins showed good retention properties for the amino-functionalized matrix. The values obtained for the equilibrium constant (K) were around 3 for BSA, both in batch reactor and column-packed operation mode. The K values for alpha -lactalbumin were even higher, 8.01 and 4.65 for the batch and column-packed experiments, respectively. Finally, axial dispersion coefficients were obtained, with a constant value of 0.0141 cm(2) s(-1) for BSA. Higher values were obtained for alpha -lactalbumin, but in this case the axial dispersion coefficient was not constant and its values were dependent on the flows used.