Three bacterial strains, which degraded azo dyes, were isolated from soil and sewage samples. The strains mere identified as Bacillus sp. OY1-2, Xanthomonas sp. NR25-2 and Pseudomonas sp. PR41-1. The bacteria produced ate-dyes-degrading enzymes. That catalyzed the reduction of methyl red and produced dimethyl p-phenylenediamine and o-aminobenzoic acid. The enzymes could thus be applied to white discharge printing of ate-dyed fabric by owing to there.