Rapid amplification of a water channel-like gene and its flanking sequences from the Methanothermobacter marburgensis genome using a single primer PCR strategy

Ding X; Kitagawa Y

Journal of Bioscience and Bioengineering, Vol.92, No.5, 488-491, 2001

Ding X, Kitagawa Y

In this study, we isolated a water channel-like gene and its flanking sequences from an archaeal genome using only a set of PCR. This method should greatly simplify gene cloning procedures and improve the efficiency of cloning.

Keywords:archaeon;water channel;single primer PCR;nested PCR;gene cloning;genomic DNA