화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.19, No.2, 261-266, March, 2002
DOI10.1007/BF02698411  Export Citation
Immobilization of GL-7-ACA Acylase for the Production of 7-ACA
Seung-Kwon Lee, Seung-Won Park1, Yong-In Kim2, Koo-Hun Chung2, Suk-In Hong1, and Seung-Wook Kim1, †
  • Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea
  • 1Department of Chemical Engineering, Korea University, Seoul 136-701, Korea
  • 2Chong Kun Dang Pharmaceutical Corporation, Seoul 120-010, Korea
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Glutaryl-7-aminocephalosporanic acid(GL-7-ACA) acylase is an important enzyme for the production of 7-ACA (7-aminocephalosporanic acid). For an efficient immobilization of GL-7-ACA acylase, various carriers were tested. A high-porous hydrophilic carrier (FPHA) among various carriers tested was found to be the best for the immobilization of GL-7-ACA acylase. In order to develop an effective immobilization method of GL-7-ACA acylase, the parameters that affect the immobilization of GL-7-ACA acylase were also investigated under different conditions of buffer solution and different concentrations of glutaraldehyde. The highest value of GL-7-ACA acylase activity (70 Unit/g-matrix) was obtained when immobilized with 1% glutaraldehyde in a 0.1M Tris buffer (pH 8.0). Also, in order to enhance the activity of the immobilized GL-7-ACA acylase, unreacted aldehyde groups were quenched by reaction with a low molecular weight agent such as L-lysine after immobilization. The highest activity of immobilized GL-7-ACA acylase was obtained at 0.1% of L-lysine. The immobilized GL-7-ACA acylase was tested for long-term stability and it was found that the activity was retained at about 62% of the initial value after 72 times of reuse at 25 ℃.
Keywords:GL-7-ACA Acylase;7-ACA;Immobilization;FPHA
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