화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.8, No.3, 203-211, May, 2002
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Partition Improvement of Cephalexin and 7-aminodeacetoxicephalospronic Acid in Aqueous Two-phase Systems for Cephalexin Synthesis
Cao Xueun, Zhu Jianhang, Wei Dongzhi, and Byung Ki Hur1, †
  • State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science and Technology, Shanghai 200237, China
  • 1Department of Biological Engineering, Inha University, Incheon 402-751, China
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Partition behavior of cephalexin and 7-ADCA in PEG-salts and EOPO-safts aqueous two-phase systems has been investigated under various different conditions. Effect of polymer molecular weight, salts types, tie line length on partition coefficients of cephalexin and 7-ADCA were described. Additives such as salts, water miscible solvents and surfactants were used to improve partition of cephalexin and 7-ADCA. NaSCN shows greatest influence on partition of cephalexin and 7-ADCA in 20% PEG 400-17.5% (NH4)(2)SO4. Cephalexin and 7-ADCA partition coefficients reached 12.92 and 2.58, respectively. While NaCl shows greatest influence on partition of cephalexin and 7-ADCA in EO40PO60-(NH4)(2)SO4. In 15% EO40PO60-13% (NH4)(2)SO4 ATPS, partition coefficients of cephalexin and 7-ADCA reached 0.08 and 0.42, respectively. Cephalexin and 7-ADCA shows different partition trend in PEG-(NH4)(2)SO4 and EOPO-(NH4)(2)SO4 ATPS. In EOPO-(NH4)(2)SO4 ATPS, diagrams of PEG3000-(NH4)(2)SO4, EO40PO60-(NH4)(2)SO4, EO20PO80-(NH4)(2)SO4 were prepared and their differences were analyzed, and recovery of EO40PO60-(NH4)(2)SO4 ATPS was performed with yield of 90-95%.
Keywords:aqueous two-phase systems;cephalexin;7-ADCA;partition
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