Biotechnology Letters, Vol.24, No.4, 275-278, 2002
Bovine serum albumin-bovine hemoglobin conjugate as a candidate blood substitute
A bovine serum albumin-bovine hemoglobin conjugate was prepared using 3-maleimidobenzoic acid N-hydroxysuccinimide ester as cross-linker. The conjugate was purified using DEAE Sepharose. It had an M-r of 127 kDa. Its P-50 (half-saturated O-2 pressure) value and Hill coefficient were 27 mm Hg and 2, respectively.
Keywords:blood substitute;bovine hemoglobin;bovine serum albumin;3-maleimidobenzoic acid N-hydroxysuccinimide;ester