Biotechnology Letters, Vol.24, No.11, 905-907, 2002
Liquid-liquid extraction of lectin from Cratylia mollis seeds using reversed micelles
Extraction of lectin from seeds of Cratylia mollis, camaratu bean, with reversed micelles of 100 mM sodium di(2-ethylhexyl) sulfosuccinate/isooctane was performed firstly with affinity-purified lectin. The best conditions were extraction of the seed extract at pH 5 and back-extraction at pH 10, giving yields of 38% and 100%, respectively.