The adsorption behavior of a hemoglobin (Hb) solution onto bentonite powder was studied from its alkaline solution (pH 12,6) and at room temperature. Different types of adsorption isotherms are discussed and various adsorption parameters, such as the adsorption coefficient, rate constants for adsorption and desorption, diffusion constant, penetration rate constant, were evaluated, The effects of pH and temperature of the adsorption medium on the extent of adsorption and the influence of solvents were studied, and the effect of salt concentrations on the rate of adsorption were also observed.