For the systhesis of new antibacterial agents, we have prepared new analogues of quinolone(10,11,12) were synthesized with cycloalkylcarbamoyl chlorides. These derivatives were synthesized via through two steps. which means, coupling at N-4 piperazne of quinolone derivatives with N-cycloalkylcarbamoylchloride provided 1-picoryl-6-fluoro-1,4-dihydro-4-oxo-7-[4-(N-cycloalkylcarbamoyl)methyl-1-pipe razinyl]-3-quinoline carboxylic acid(10,11,12) in yield of 84.2%, 87.8%, and 91.5% respectively. and These compounds were tested in vitro on antibacterial activity against Gram-positive and Gram-negative bacteria including Pseudomonas Aeruginost ATCC9027.