Chromatographic Analysis of Ceramide III of Saccharomyces cerevisiae

Mi Soon Yeo; Duk Hui Kang; Se Kyung Kim; Hyun Shik Yun; Yoon Mo Koo; Youn-Woo Lee; Kyung Ho Row

Korean Journal of Chemical Engineering, Vol.19, No.5, 815-817, September, 2002

Mi Soon Yeo¹, Duk Hui Kang¹, Se Kyung Kim^{1, 2}, Hyun Shik Yun¹, Yoon Mo Koo¹, Youn-Woo Lee³, and Kyung Ho Row^{1, 2, †}

¹Center for Advanced Bioseparation Technology, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea
²Div. of Chem. Eng., Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea
³National Research Lab. for Supercritical Fluid, KIST, Seoul 136-791, Korea

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Ceramide was prepared by the cultivation of Saccharomyces cerevisiae from cell extracts by solvent extraction and analyzed by NP-HPLC using a UV detector. The mobile phase was composed of hexane, methanol, and IPA. From the experimental conditions, the composition of mobile phase was 72/5/23 (hexane/IPA/methanol, vol%). Quantitative analysis of ceramide was performed. Based on the analytical conditions, the effect of cultivation temperature for the production of ceramide was investigated and the optimum cultivation temperature was found to be 35 ℃.

Keywords:Ceramide;Saccharomyces cerevisiae;Preparative HPLC

[References]

Bouwstra JA, Cheng K, Gooris GS, Weerheim A, Ponec M, Biochim. Biophys. Acta, 1300, 177 (1996)
Dickson RC, Lester RL, Biochim. Biophys. Acta, 1426, 347 (1999)
Gildenast T, Lasch J, Biochim. Biophys. Acta, 1346, 69 (1997)
Kolesnick RN, Goni FM, Alonso A, J. Cell. Phys., 184, 285 (2000)
Lee JW, Row KH, Biotech. Bioproc. Eng., 6(1), 371 (2001)
Lewis T, Nichols PD, Mcmeekin TA, J. Microbiol. Methods, 43, 107 (2000)
Long AS, Wertz PW, Strauss JS, Downing DT, Arch. Derm. Res., 277, 284 (1985)
Patton JL, Lester RL, J. Bacteriology, 173, 3101 (1991)
Raith K, Neubert RHH, Anal. Chim. Acta, 403, 295 (2000)
Raith K, Darius J, Neubert RHH, J. Chromatogr. A, 876, 229 (2000)
Robson KJ, Stewart ME, Michelsen S, Lazo ND, Downing DT, J. Lipid Res., 35, 2060 (1994)
Rupcic J, Maric V, Chem. Phys. Lipids, 91, 153 (1998)
Rupcic J, Mesaric M, Maric V, Appl. Microbiol. Biotechnol., 50(5), 583 (1998)
Zhou JY, Chaminade P, Gaudin K, Prognon P, Baillet A, Ferrier D, J. Chromatogr. A, 859, 99 (1999)

[Related Articles]

[1] Bouwstra JA, Cheng K, Gooris GS, Weerheim A, Ponec M, Biochim. Biophys. Acta, 1300, 177 (1996)

[2] Dickson RC, Lester RL, Biochim. Biophys. Acta, 1426, 347 (1999)

[3] Gildenast T, Lasch J, Biochim. Biophys. Acta, 1346, 69 (1997)

[4] Kolesnick RN, Goni FM, Alonso A, J. Cell. Phys., 184, 285 (2000)

[5] Lee JW, Row KH, Biotech. Bioproc. Eng., 6(1), 371 (2001)

[6] Lewis T, Nichols PD, Mcmeekin TA, J. Microbiol. Methods, 43, 107 (2000)

[7] Long AS, Wertz PW, Strauss JS, Downing DT, Arch. Derm. Res., 277, 284 (1985)

[8] Patton JL, Lester RL, J. Bacteriology, 173, 3101 (1991)

[9] Raith K, Neubert RHH, Anal. Chim. Acta, 403, 295 (2000)

[10] Raith K, Darius J, Neubert RHH, J. Chromatogr. A, 876, 229 (2000)

[11] Robson KJ, Stewart ME, Michelsen S, Lazo ND, Downing DT, J. Lipid Res., 35, 2060 (1994)

[12] Rupcic J, Maric V, Chem. Phys. Lipids, 91, 153 (1998)

[13] Rupcic J, Mesaric M, Maric V, Appl. Microbiol. Biotechnol., 50(5), 583 (1998)

[14] Zhou JY, Chaminade P, Gaudin K, Prognon P, Baillet A, Ferrier D, J. Chromatogr. A, 859, 99 (1999)