Laser-induced fluorescence of jet-cooled biacetyl was recorded in the region of 22 177-22 277 cm-1 with a resolution of 0.012 cm-1. Three bands, 0(0)(0), 15(0)(1), and 21(0)(1) with transitions J(')less than or equal to3 were observed and analyzed. For the 0(0)(0) band, E-2-E-1 and G-G subbands were observed. However, only c-type transitions, DeltaK(a)=odd, DeltaK(c)=even, were observed. Another kind of transition, d-type (DeltaK(a)=even, DeltaK(c)=even), which is allowed by selection rules, was not observed. For the 15(0)(1) band, two subbands E-3-E-3 and G-G were observed. The E-3-E-3 subband can be described by the rigid rotor model, and it contains only DeltaK(a)=odd, DeltaK(c)=even transitions. On the other hand, the G-G subband cannot be even approximately described by the rigid rotor model. It is dominated by c-type transitions. In addition, a/b-type and d-type transitions were all observed, though the intensities are small. For the 21(0)(1) band, E-2-E-1, G-G, and A(3)-A(1) subbands were observed. The E-2-E-1, G-G subbands contain all the possible types of transitions, i.e., a/b-and c/d-type transitions. However, the A(3)-A(1) subband has DeltaK(a)=odd, DeltaK(c)=even transitions only. The band origin, rotational constants, and the splitting due to the tunneling in these three bands was accurately measured. Relatively large vibronic transition intensities were observed in both the 15(0)(1) and 21(0)(1) bands.