Journal of Physical Chemistry B, Vol.106, No.34, 8489-8492, 2002
Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes Raman scattering (CARS) near-field scanning optical microscopy (NSOM)
We demonstrate the combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) for chemically selective imaging via intrinsic vibrational resonances with spatial resolution below the diffraction limit. Femtosecond, near-IR pulses are used to produce CARS signals from human hepatocytes, and by tuning the CARS signal to be resonant with C-H stretching frequencies, image contrast is observed with an optical spatial resolution of similar to128 nm.