Tetraploa aristata CLPS 419 produced maximum extracellular laccase activity at over 9 mU ml(-1) in shaking cultures supplemented with glucose and 3.5 mU ml(-1) in sucrose-grown ones. Laccase activity did not exceed 0.7 mU ml(-1) in stationary cultures with glucose and was not detected in similar cultures with sucrose or in ones grown on lignin.