Theoretical Study for the Separation of Polyelectrolytes by Electrophoresis in the Chromatography

Young G. Park; Hwa E. Lim

Journal of Industrial and Engineering Chemistry, Vol.8, No.5, 419-426, September, 2002

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Theoretical Study for the Separation of Polyelectrolytes by Electrophoresis in the Chromatography

Young G. Park^†, and Hwa E. Lim

Department of Chemical Eng., Daejin University, Gyungkido 487-711, Korea

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Electrophoretic separation was theoretically performed for large polyelectrolytes in the column packed with polymeric gel particles. This paper dealed with the computer simulation based on the Langevin equation of motion and the governing equation for the mass transfer in the presence of an electric field, the theoretical calculation of migration of polyelectrolyte in gel contributed to the understandings of mass transfer in the column. Theoretical studies were investigated in order to establish a relationship providing intraparticle convection effects as a function of an electric field, since a polyelectrolyte quickly orients through the pores of gel in the field direction. Dimensionless transient equations were derived considering mobility for the separation of polyelectrolytes and they were studied by two different dimensionless numbers in the fluid and solid phases in the column, showing how the concentration profiles of polyelectrolyte inside a particle varied in the column according to values of dimensionless number of individual polyelectrolyte. Langevin and governing equations were solved using the numerical method and an operator theory.

Keywords:electrophoresis;electrophoretic velocity;polyelectrolyte;Rouse chain

[References]

Frey DD, Schweinheim E, Horvath C, Biotechnol. Prog., 9, 273 (1993)
Park Y, J. Biochem. Eng., 7, 213 (2001)
Kang DH, Row KH, J. Ind. Eng. Chem., 7(3), 178 (2001)
Cole KD, Cabezas H, J. Chromatogr. A, 760, 259 (1997)
Slater GW, Noolandi J, Biopolymer, 25, 431 (1986)
Ramkrishna D, Amundson N, Linear Operator Methods in Chemical Engineering with Applications to Transport and Chemical Reaction Systems, Prentice-Hall, Englewood Cliffs, NJ U.S.A. (2000)
Doi M, Edwards SF, The Theory of Polymer Dynamics, Oxford University Press, Oxford, Great Britain (1986)
Cho D, J. Ind. Eng. Chem., 6(5), 325 (2000)
Locke B, Arce P, Park Y, Chem. Eng. Sci., 48, 4007 (1993)
Park JY, Han SW, Son SY, Song SH, Kim JM, Ohtani T, Muramatsu H, Chang SM, HWAHAK KONGHAK, 40(1), 100 (2002)
Maier B, Radler JO, Macromolecules, 33(19), 7185 (2000)
Pecora P, Science, Feb., 893 (1991)
Dejardin P, Lumpkin OJ, Zimm B, J. Polym. Sci., 73, 67 (1985)

[Referenced By]

[1] Frey DD, Schweinheim E, Horvath C, Biotechnol. Prog., 9, 273 (1993)

[2] Park Y, J. Biochem. Eng., 7, 213 (2001)

[3] Kang DH, Row KH, J. Ind. Eng. Chem., 7(3), 178 (2001)

[4] Cole KD, Cabezas H, J. Chromatogr. A, 760, 259 (1997)

[5] Slater GW, Noolandi J, Biopolymer, 25, 431 (1986)

[6] Ramkrishna D, Amundson N, Linear Operator Methods in Chemical Engineering with Applications to Transport and Chemical Reaction Systems, Prentice-Hall, Englewood Cliffs, NJ U.S.A. (2000)

[7] Doi M, Edwards SF, The Theory of Polymer Dynamics, Oxford University Press, Oxford, Great Britain (1986)

[8] Cho D, J. Ind. Eng. Chem., 6(5), 325 (2000)

[9] Locke B, Arce P, Park Y, Chem. Eng. Sci., 48, 4007 (1993)

[10] Park JY, Han SW, Son SY, Song SH, Kim JM, Ohtani T, Muramatsu H, Chang SM, HWAHAK KONGHAK, 40(1), 100 (2002)

[11] Maier B, Radler JO, Macromolecules, 33(19), 7185 (2000)

[12] Pecora P, Science, Feb., 893 (1991)

[13] Dejardin P, Lumpkin OJ, Zimm B, J. Polym. Sci., 73, 67 (1985)