The filterability of activated sludge is an important factor for the economical operation of membrane bioreactors (MBR). In the literature mainly investigations on sludge dewaterability in respect to further disposal are published. In this study, a procedure for determining filterability in a crossflow test cell is introduced. Its features are: no increase in sludge concentration during batch trials, crossflow conditions, and little impact on the sludge structure. The activated sludge filterability is given as the ratio of permeate flux after 40 min of operation to clear water flux. Sludge samples of eight different MBR and one conventional wastewater treatment plant (wwtp) have been examined and compared. Contrary to the literature, no impact of suspended solids (SS), concentration, sludge viscosity or extractable extracellular polymer substances (EPS) concentration on the filterability was found. Instead, the composition of the liquid phase was found to effect most the filterability of activated sludge, a major influence being the concentration of suspended EPS: the higher the suspended EPS concentration, the lower the filtration index. Suspended EPS concentration increases with high mechanical stress in the MBR and high F/M ratios, if the treated wastewater contains considerable amounts of proteins or polysaccharides.