The agro-industrial byproducts from the industries for the Sikhye, traditional Korean food production (AIB-I). the apple juice production (AIB-II). and the soy sauce production (AIB-III) were examined as the substrate for the production of the heteropolysaccharide (PS-7) by B. indica HS2001. Major components of AIB-I are dextrin. maltotriose. and maltose. Major ones of AIB-II and AIB-II were carbohydrate and protein. respectively. Dextrin and AIB-II were found to be better carbon sources than glucose for the production of PS-7. Conversion rates of PS-7s front 2% (w/v) dextrin and 2% (w/v) AIB-II as the carbon source were 20%. As concentration of agro-industrial byproducts as the carbon source increased, cell growth and the production of PS-7 increased whereas conversion rates of PS-7s decreased. The monomeric components of PS-7s produced by B. indica HS2001 with agro-industrial byproducts as the carbon source were identified to be glucose and rhamnose and their molar ratios of rhamnose to glucose were all in the range of 1.0 to 4.5similar to4.7. It seems that B. indica HS2001 utilized all the agro-industrial byproducts used in this study and produced PS-7s with the same composition as well as the same molar ratio of their monomeric component.