We report a simple preparation method for TiO2 particles dispersed activated carbons and their photo-sterilization activity for E. coli and B. subtilis. The TiO2 particles dispersed activated carbons were readily prepared by steam activation of pitch containing titanium isopropoxide Ti(OiPr)(4) or titanium acetylacetonate TiO(acac)(3). The resulting activated carbons have high BET surface area and fine TiO2 particles are uniformly dispersed. The photo-sterilization activities for E. coli and B. subtilis were estimated by the sterilization activity for bacteria spores by irradiation of black light to the solutions containing activated carbons. The obtained activated carbons exhibited high photo-sterilization activity for E. coli and B. subtilis with short irradiation time of 15 min.