Fluidized bed chromatography is emerging as a powerful tool in bioseparation of proteins/enzymes. In this work, alginate and chitosan beads have been used as affinity media in fluidized mode to selectively capture alpha-amylases and Aspergillus niger cellulase, respectively. Fairly high yields (>80%), with homogeneity at the level of single band on sodium dodecyl sulfate - polyacrylamide gel electrophoresis, were achieved in all these cases. The main novel features are unexpected affinity of the polysaccharides towards the target enzymes; such macroaffinity ligands avoid any costly chemical steps for affinity ligands; and possibility of easy scale up in fluidized bed mode and single step purification protocols.