Journal of Bioscience and Bioengineering, Vol.94, No.1, 84-86, 2002
In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis
A mutant library of Burkholderia cepacia lipase KWI-56 was constructed on microplates by a cell-free process and tested with a chromogenic assay. This high-throughput construction system can be used to screen mutant proteins based on their catalytic activity.
Keywords:Burkholderia cepacia;in vitro protein synthesis;lipase;protein engineering;protein library construction