A peptone for utilization in microbiological culture media can be produced from rams horns. A major component of the horns is protein. Peptones are defined as protein hydrolysates. Ram horns obtained from a slaughterhouse in Erzurum, Turkey were hydrolyzed by treatment with acid (6N-HCl) and ram horn hydrolysate (RHH) was obtained. The RHH was evaporated and termed ram horn peptone (RHP). RHP was compared with a bacto-tryptone from casein and other peptones. The results show that RHP can be utilized as a peptone and may be a valuable supplement in biotechnology.