Overexpression and purification of microbial pro-transglutaminase from streptomyces cinnamoneum and in vitro processing by Streptomyces albogriseolus proteases

Taguchi S; Arakawa K; Yokoyama K; Takehana S; Takagi H; Momose H

Journal of Bioscience and Bioengineering, Vol.94, No.5, 478-481, 2002

Taguchi S, Arakawa K, Yokoyama K, Takehana S, Takagi H, Momose H

Processing and activation of the precursor of an extracellular Streptomyces transglutaminase were achieved by using three Streptomyces proteases (SAM-P20, SAM-P26 and SAM-P45), all of which are widely distributed in Streptomyces. The use of these proteases would allow us to develop a production process for the active form of this enzyme in recombinant bacteria.

Keywords:transglutaminase;pro-region;Streptomyces cinnamoneum;Streptomyces albogriseolus;protease;processing