An inverse problem for turbulent forced convection between parallel flat plates is investigated. The space- and time-dependent heat flux at the upper wall is estimated from the temperature measurements taken inside the flow. In the present study, the conjugate gradient method is adopted for the estimation of the unknown wall heat flux. No prior information is needed for the functional form of the wall heat flux in the inverse analysis. The effects of the measurement errors, the functional form of the wall heat flux, and the location of the sensors on the accuracy of the estimation are investigated. The reconstruction of the wall heat flux is satisfactory when simulated exact or noisy data are input to the inverse analysis. The sensitivity coefficients are discussed in this paper. As expected, it is shown that the accuracy of the estimation can be improved when the sensors are located closer to the upper wall.