In this work a system which consists of chitosan microcores entrapped in ethylcellulose is presented. Vitamin D-2 was efficiently entrapped in chitosan microcores with spray-drying method and was microencapsulated by coating of ethylcellulose. The average size of chitosan microspheres was 6.06 mum. The morphology and release properties of microcapsules were tested. The results of release in vitro showed that the microcapsule could realize sustained release for 12 h in artificial intestinal juice.