Introduction of the archaebacterial geranylgeranyl pyrophosphate synthase gene into Chlamydomonas reinhardtii chloroplast

Fukusaki EI; Nishikawa T; Kato K; Shinmyo A; Hemmi H; Nishino T; Kobayashi A

Journal of Bioscience and Bioengineering, Vol.95, No.3, 283-287, 2003

Fukusaki EI, Nishikawa T, Kato K, Shinmyo A, Hemmi H, Nishino T, Kobayashi A

Geranylgeranyl pyrophosphate (GGPP) synthase gene (gds) derived from a thermophilic Archae Sulfolobus acidocaldarius, was introduced into a unicellular green alga Chlamydomonas reinhardtii chloroplast. Heat treatment abolished the prenyltransferase activity of the wild strain, but the activity of the transformant remained. The transformant accumulated gds gene mRNA and translation product.

Keywords:terpenoid;polyisoprene;prenyltransferase;Chlamydomonas;geranylgeranyldiphosphate synthase;chloroplast