Phase Behaviors of Binary Protein Systems: Consideration of Structural Effects

Sang Gon Kim; Sung Ho Kong; Young Chan Bae; Sunjoon Kim

Macromolecular Research, Vol.11, No.4, 241-249, August, 2003

Export Citation

Phase Behaviors of Binary Protein Systems: Consideration of Structural Effects

Sang Gon Kim, Sung Ho Kong , Young Chan Bae^†, and Sunjoon Kim¹

Division of Chemical Engineering, Hanyang University, Seoul 133-791, Korea
¹Department of Environmental Geosystem Engineering, Hanyang University, Seoul 133-791, Korea

E-mail:

A molecular-thermodynamic model to describe the salt-induced protein precipitation is developed based on the perturbation theory. We employed the modified perturbed hard-sphere-chain (PHSC) equation of state for copolymer mixtures to take into account the pre-aggregation effect among protein particles. Hypothetical pressure-composition diagrams are computed with various size differences and salt concentrations. The precipitation behaviors are also studied for various types of pre-aggregation effect for the given systems.

Keywords:pre-aggregation;binary protein system;interaction potentials;phase equilibria

[References]

Foster PR, Dunhill P, Lilly MD, Biochem. Biophys. Acta, 317, 505 (1975)
Haire RN, Tisel WA, White JC, Rosenberg A, Biopolymers, 23, 2761 (1984)
Shih YC, Blanch HW, Prausnitz JM, Biotechnol. Bioeng., 40, 1155 (1992)
Niederauer MQ, Glatz CE, Adv. Biochem. Eng. Technol., 47, 159 (1992)
Rothstein F, Protein Precipitation Process Engineering, R.G. Harrion, Ed., Dekker, New York (1994)
Belter PA, Cussler EL, Hu WS, Bioseparations: Downstream Processing Operations, Wiley, New York (1998)
Rothstein F, Protein Purification Process Engineering, R.G. Harvision, Ed., Macel Dekker, New York (1994)
Coen CJ, Blanch HW, Prausnitz JM, AIChE J., 41(4), 996 (1995)
Wu JZ, Prausnitz JM, Fluid Phase Equilib., 155(1), 139 (1999)
Verwey E, Overbeek J, Theory of Stability of Lyophobic Colloids, Elsevier, Amsterdam (1948)
Askura S, Oosawa F, J. Polym. Sci., 33, 183 (1958)
Vrij A, Pure Appl. Chem., 48, 471 (1976)
Joanny JF, Leibler L, deGennes PG, J. Polym. Sci. B: Polym. Phys., 17, 1073 (1979)
DeHek H, Vrij A, J. Colloid Interface Sci., 41, 996 (1995)
Gast AP, Hall CK, Russel WG, J. Farad. Discuss. Chem Soc., 76, 189 (1983)
Grimson MJ, J. Chem. Soc.-Faraday Trans., 79, 817 (1983)
Victor JM, Hansen JP, J. Phys. Lett., 45, 307 (1984)
Mahadevan H, Hall CK, AIChE J., 36, 1517 (1990)
Mahadevan H, Hall CK, AIChE J., 38, 573 (1992)
Vlachy V, Prausnitz JM, J. Phys. Chem., 96, 6465 (1992)
Vlachy V, Blanch HW, Prausnitz JM, AIChE J., 39, 215 (1993)
Chiew YC, Mol. Phys., 70, 129 (1990)
Kuehner DE, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 116(1-2), 140 (1996)
Kim SG, Bae YC, Ryu SO, Korean J. Chem. Eng., 17(6), 638 (2000)
Song YH, Lambert SM, Prausnitz JM, Macromolecules, 27(2), 441 (1994)
Song YH, Lambert SM, Prausnitz JM, Ind. Eng. Chem. Res., 33(4), 1047 (1994)
Song YH, Lambert SM, Prausnitz JM, Chem. Eng. Sci., 49(17), 2765 (1994)
Hino T, Song YH, Prausnitz JM, Macromolecules, 27(20), 5681 (1994)
Hino T, Song YH, Prausnitz JM, J. Polym. Sci. B: Polym. Phys., 34(12), 1977 (1996)
Carnahan NF, Starling KE, J. Chem. Phys., 51, 635 (1969)
Young DA, J. Chem. Phys., 98, 9819 (1993)
Albertsson PA, Partition of Cell Particles and Macromolecules, Wiley, New York (1986)
Hamaker HC, Physica IV, 10, 1058 (1937)
Kuehner D, Heyer C, Ramsch C, Fornefeld UM, Blanch HW, Prausnitz JM, Biophys. J., 73, 3211 (1997)
Curtis RA, Steinbrecher C, Heinemann M, Blanch HW, Prausnitz JM, Biophys. Chem., 98, 249 (2002)
Kim SG, Bae YC, Macromol. Res., 11(1), 53 (2003)

[Referenced By]

[Related Articles]

[1] Foster PR, Dunhill P, Lilly MD, Biochem. Biophys. Acta, 317, 505 (1975)

[2] Haire RN, Tisel WA, White JC, Rosenberg A, Biopolymers, 23, 2761 (1984)

[3] Shih YC, Blanch HW, Prausnitz JM, Biotechnol. Bioeng., 40, 1155 (1992)

[4] Niederauer MQ, Glatz CE, Adv. Biochem. Eng. Technol., 47, 159 (1992)

[5] Rothstein F, Protein Precipitation Process Engineering, R.G. Harrion, Ed., Dekker, New York (1994)

[6] Belter PA, Cussler EL, Hu WS, Bioseparations: Downstream Processing Operations, Wiley, New York (1998)

[7] Rothstein F, Protein Purification Process Engineering, R.G. Harvision, Ed., Macel Dekker, New York (1994)

[8] Coen CJ, Blanch HW, Prausnitz JM, AIChE J., 41(4), 996 (1995)

[9] Wu JZ, Prausnitz JM, Fluid Phase Equilib., 155(1), 139 (1999)

[10] Verwey E, Overbeek J, Theory of Stability of Lyophobic Colloids, Elsevier, Amsterdam (1948)

[11] Askura S, Oosawa F, J. Polym. Sci., 33, 183 (1958)

[12] Vrij A, Pure Appl. Chem., 48, 471 (1976)

[13] Joanny JF, Leibler L, deGennes PG, J. Polym. Sci. B: Polym. Phys., 17, 1073 (1979)

[14] DeHek H, Vrij A, J. Colloid Interface Sci., 41, 996 (1995)

[15] Gast AP, Hall CK, Russel WG, J. Farad. Discuss. Chem Soc., 76, 189 (1983)

[16] Grimson MJ, J. Chem. Soc.-Faraday Trans., 79, 817 (1983)

[17] Victor JM, Hansen JP, J. Phys. Lett., 45, 307 (1984)

[18] Mahadevan H, Hall CK, AIChE J., 36, 1517 (1990)

[19] Mahadevan H, Hall CK, AIChE J., 38, 573 (1992)

[20] Vlachy V, Prausnitz JM, J. Phys. Chem., 96, 6465 (1992)

[21] Vlachy V, Blanch HW, Prausnitz JM, AIChE J., 39, 215 (1993)

[22] Chiew YC, Mol. Phys., 70, 129 (1990)

[23] Kuehner DE, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 116(1-2), 140 (1996)

[24] Kim SG, Bae YC, Ryu SO, Korean J. Chem. Eng., 17(6), 638 (2000)

[25] Song YH, Lambert SM, Prausnitz JM, Macromolecules, 27(2), 441 (1994)

[26] Song YH, Lambert SM, Prausnitz JM, Ind. Eng. Chem. Res., 33(4), 1047 (1994)

[27] Song YH, Lambert SM, Prausnitz JM, Chem. Eng. Sci., 49(17), 2765 (1994)

[28] Hino T, Song YH, Prausnitz JM, Macromolecules, 27(20), 5681 (1994)

[29] Hino T, Song YH, Prausnitz JM, J. Polym. Sci. B: Polym. Phys., 34(12), 1977 (1996)

[30] Carnahan NF, Starling KE, J. Chem. Phys., 51, 635 (1969)

[31] Young DA, J. Chem. Phys., 98, 9819 (1993)

[32] Albertsson PA, Partition of Cell Particles and Macromolecules, Wiley, New York (1986)

[33] Hamaker HC, Physica IV, 10, 1058 (1937)

[34] Kuehner D, Heyer C, Ramsch C, Fornefeld UM, Blanch HW, Prausnitz JM, Biophys. J., 73, 3211 (1997)

[35] Curtis RA, Steinbrecher C, Heinemann M, Blanch HW, Prausnitz JM, Biophys. Chem., 98, 249 (2002)

[36] Kim SG, Bae YC, Macromol. Res., 11(1), 53 (2003)