Biotechnology Letters, Vol.25, No.16, 1325-1328, 2003
Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates
The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.