방사선 가교에 의한 폴리(비닐 피롤리돈)/키토산 수화겔의 제조와 약물방출 효과

김현우; 노영창; 손태일; Hyun-Woo Kim; Young-Chang Nho; Tae-Il Son

Journal of the Korean Industrial and Engineering Chemistry, Vol.15, No.3, 283-287, May, 2004

Export Citation

방사선 가교에 의한 폴리(비닐 피롤리돈)/키토산 수화겔의 제조와 약물방출 효과

Antibiotic Delivery Characteristic of Poly(vinyl pyrrolidone)/chitosan Hydrogel Prepared by Irradiation

김현우^†, 노영창 , 손태일¹

한국원자력연구소 동위원소/방사선 응용연구팀, 대전 305-600
¹중앙대학교 생명공학과, 경기 456-756

Hyun-Woo Kim^†, Young-Chang Nho , and Tae-Il Son¹

Radioisotopes/Radiation Application Team, Korea Atomic Energy Research Institute, Daejeon 305-600, Korea
¹Department of Biotechnology, Chung-Ang University, Kyonggi-do 456-756, Korea

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초록

약물방출 기작은 인체 내의 치주, 입, 위 그리고 장과 같이 산도(pH)가 서로 다른 인체부위에서도 조절이 되도록 발전되어왔다. 이와 같이 pH에 따라 특성을 달리하는 수화겔은 대부분 생물의학제재 및 약물방출 시스템의 조절에 이용된다. 본 연구는 분자량 4 × 10⁴ 및 3.6 × 10⁵의 폴리비닐피롤리돈(Poly vinyl pyrrolidone; PVP)과 수용성 키토산을 혼합하여 ⁶⁰Co의 감마선을 이용하여 수화겔을 제작하였다. 이 수화겔을 37 ℃의 pH 2, 7에서 팽윤특성을 관찰하였다. 항생제를 탑재한 drug-gel은 자연건조법(air drying)과 동결건조법(freezing dry)으로 각각 건조시켜 제조하였다. Drug-gel의 in vitro에서의 아목시실린 방출은 자외선분광기를 사용하여 분석하였다. 약물방출실험은 다양한 수화겔을 사용하여 37 ℃의 인공위액(simulated gastric fluid; SGF)과 인공장액(simulated intestinal fluid; SIF)에서 실시하였다.

Controlled release systems have been developed over a range of pH-domains in the body, e.g. for the periodontal, oral, gastric and intestinal applications. These pH-sensitive hydrogels are of general interest for biomedical applications and controlled drug release systems. In this study the poly(vinyl pyrrolidone) (PVP)/chitosan hydrogels were synthesized by crosslinking PVP (MW 4 × 10⁴ and 3.6 × 10⁵) and soluble chitosan blend using γ-rays with ⁶⁰Co source at room temperature, their swelling behavior was examined in pH 2 and 7 at 37 ℃. The drug-gels were prepared by the air drying and freezeing day methods after loading amoxicillin as antibiotics. The amoxicilling release of the drug-gels in vitro was examined by UV analysis. It was found that the basic parameters affecting the dug release behavior of PVP/chitosan hydrogels are the pH of the simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) at 37 ℃.

Keywords:amoxicillin;chitosan;hydrogel;poly(vinyl pyrrolidone)

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[Related Articles]

[1] Jeong B, Choi YK, Bae YH, Zentner G, Kim SW, J. Control. Release, 62, 109 (1999)

[2] Kim YH, Bae YH, Kim SW, J. Control. Release, 28, 143 (1994)

[3] Mirejovsky D, Patel AS, Young G, Biomaterials, 14, 1080 (1993)

[4] Ratner BD, J. Biomed. Mater. Res., 28, 837 (1993)

[5] Esposito E, Cortesi R, Nastruzzi C, Biomaterials, 17, 2009 (1996)

[6] Pascual B, Castellano I, Vazquez B, Gurruchaga M, Goni I, Polymer, 37(6), 1005 (1996)

[7] Vazquez B, Gurruchaga M, Goni I, Polymer, 36(11), 2311 (1995)

[8] Munjeri O, Collett JH, Fell JT, J. Control. Release, 46, 273 (1997)

[9] Jameela SR, Jayakrishnan A, Biomaterials, 16, 769 (1995)

[10] Araki Y, Ito E, Eur. J. Biochem., 55, 71 (1975)

[11] Tarsi R, Corbin B, Pruzzo C, Muzzarelli RA, Oral Microbiol. Immunol., 13, 217 (1998)

[12] Siegel RA, Falamarzian M, Firestone BA, Moxley BC, J. Control. Release, 8, 179 (1988)

[13] Sakurai K, Maegawa T, Takahashi T, Polymer, 41(19), 7051 (2000)

[14] Risbud MV, Hardikar AA, Bhat SV, Bhonde RR, J. Control. Release, 68, 23 (2000)

[15] Patel VR, Arniji MM, Pharm. Res., 13, 588 (1996)

[16] Rosiak JM, Ulanski P, Radiat. Phys. Chem., 55, 139 (1999)

[17] Shantha KL, Harding DRK, Int. J. Pharm., 207, 65 (2000)

[18] Sen M, Guven O, Radiat. Phys. Chem., 55, 113 (1999)